Figure 2.

FKBP51 is SUMOylated at lysine 422. (a) Putative SUMOylation sites with the highest probability. (b) Schematic representation of FKBP51: FK1, FK2 and TPR domains. The two putative SUMO-attachment sites are depicted. FKBP51 sequences are aligned centered on human K138 and K422. SUMOylation motifs are boxed. (c) HT22 cells were transfected with the indicated plasmids. SUMOylated proteins were purified by Ni²⁺ affinity chromatography and analyzed by western blotting using anti-FKBP51 antibody. *Non-specific. (d) Pull-down assays were performed using HEK293T lysates expressing wt, K422R or 2KR FLAG-FKBP51 and analyzed by western blotting using anti-FKBP51 antibody