Figure 3.

Knockdown of PTBP1 stabilizes MCL1 mRNA. PC3 and H1299 cells transfected with either siControl or a mixture of two siPTBP1 for 48 h were treated with Act. D for indicated intervals. MCL1 mRNA decay was assessed by RT-qPCR using GAPDH as the internal control. (a) Data are represented as a log10 plot of the percentage of MCL1 mRNA remaining versus the time of incubation with Act. D from one representative RT-qPCR experiment. (b) MCL1 mRNA half-life (t1/2) was calculated from three independent experiments. All data are shown as mean±S.E.M., n=3 or more. The statistical significance was determined by unpaired student t-test where *P<0.05; **P<0.01; ***P<0.001