Figure 6.

Knockdown of PTBP1 increases cell viability through MCL1 under mitotic stress. PC3 cells transfected with either siControl or a mixture of two siPTBP1 were treated with 1000 nM docetaxel (a) or vinscristine (b) for 48 h. Cell morphology was checked by phase contrast microscope (scale bar, 100 μm). The morphology of untreated PC3 cells (c) were shown for comparison (scale bar, 100 μm). (d) PC3 cells were transfected with siControl; a mixture of two siPTBP1; a mixture of two siPTBP1 and two siMCL1; or a mixture of two siMCL1. Cell viability was assessed by MTS assay with various concentrations of docetaxel or vincristine for 48 h. The viability of cells treated with DMSO was set as 100%. (e) Abs (490 nm) was measured by MTS assay in siControl or siPTBP1 treated PC3 cells with DMSO, 1000 nM ABT737, or 100 nM Sabutoclax for 48 h, showing no significant change in cell viability following siPTBP1 treatment in DMSO control, ABT737, or Sabutoclax treated cells. (f) PC3 cells transfected with siControl or a mixture of two siPTBP1 were treated with 1000 nM ABT737 or 100 nM Sabutoclax combined with various doses of docetaxel for 48 h and cell viability was assessed by MTS assay. The viability of cells with 1000 nM ABT737 or 100 nM Sabutoclax alone was set as 100%. All data are presented as mean±S.E.M., n=3. The statistical significance was determined by unpaired student t-test where *P<0.05; **P<0.01; ***P<0.001