Figure 5.

Metabolic state of Casp2^−/− mice favours tumour progression. Reduced (a) glycogen levels, (b) glucose-6-phosphate levels and (c) G6PDH activity in DEN-injected Casp2^−/− mice. Decrease in Sirt1 (d) and Sirt3 (e) activities in DEN-injected Casp2^−/− mice liver 10 months after PBS/DEN administration. Values are given as mean±S.E.M. (n=6, PBS and n=11, DEN). Statistics were performed using Student's t-test: *P<0.05 and **P<0.01. (f) Immunoblot analysis showing increased Sirt1 protein expression in DEN-injected Casp2^−/− mice. Upregulation of p53 and its downstream effector p21 was also observed