Fig. 2.

a) Immune responses in mice immunized s.c. or i.p. with OVA + CAF09 three times with two-week intervals, assessed eight days after the final immunization. Percentage of antigen-specific CD8⁺ T cells in the blood identified as SIINFEKL⁺ CD44^hi CD8⁺ T-cells, as described in Supplementary Data Fig. S1. Data points represent individual mice and mean values, n = 4–8. b–d) Recovery of radiolabelled vaccine components [³H-liposomes, ³²P-poly(I:C) and ¹²⁵I-OVA] as the fraction of the administered dose at the SOI (b), the dLN (c) and the spleen (d) 6 h after administration. Data points represent mean values + SEM (n = 4). *p ≤ 0.05, ***p ≤ 0.001, ****p ≤ 0.0001.