Fig. 4.

Qualitative association of fluorescently labelled vaccine components with DCs/monocytes was evaluated as described in Supplementary data, Fig. S2. a) Mice were immunized with unadj. OVA or OVA + CAF09, respectively, either i.p. (left) or i.m. (right), and the percentage of OVA⁺ monocytes in the dLNs was evaluated. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 for each time point. Data points represent mean ± SEM (n = 8–12). b) MFI values determined for OVA⁺ monocytes in the dLNs. *p ≤ 0.05, ***p ≤ 0.001, within each time point. Data points represent mean ± SEM (n = 6–12). c) The percentage of OVA⁺ monocytes in the spleen following i.p. (left) and i.m. (right) immunization. *p ≤ 0.05, **p ≤ 0.01 for each time point. Data points represent mean ± SEM (n = 8–12). d) Maturation of DCs (CD86⁺) in the dLNs as response to immunization with unadj. OVA and OVA + CAF09, respectively, either i.p. (left) or i.m. (right). **p ≤ 0.01, ****p ≤ 0.0001, of the OVA + CAF09 group compared to all other groups for that time point. No markers: No significance. Data points represent mean ± SEM (n = 4). The data are also presented in Fig. S5 as the number of cells.