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. 2016 Oct 10;239:107–117. doi: 10.1016/j.jconrel.2016.08.034

Fig. 6.

Fig. 6

Activation of T cells in the dLNs and the spleen following i.p. immunization with unadj. OVA, OVA + CAF04, and OVA + CAF09. a) Fraction of CD69+ CD4+ T-cells in the dLNs following immunization. Data represent mean ± SEM (n = 3–4). ****p ≤ 0.0001, of the unadj. OVA group compared to all other groups within that time point. No marker: No significance. b) Fraction of CD69+, CD8+ T cells in the dLNs following immunization. Data represent mean ± SEM (n = 3–4). ****p ≤ 0.001, of the OVA + CAF09 group, as compared to all other groups at 6 h, and ****p ≤ 0.0001, of the unadj. OVA group compared to all other groups within that time point. No marker: No significance. c) Fractions of CD69+ CD4+ T-cells in the spleen following immunization. Data represent mean ± SEM (n = 3–4). ####p ≤ 0.0001, of the OVA + CAF04 group, as compared to unadj. OVA at 24 h, ****p ≤ 0.0001, of the OVA + CAF09 group, as compared to all other groups at 24 h, and ***p ≤ 0.001, of the OVA + CAF09 group, as compared to all other groups at 48 h. No marker: No significance. d) Fractions of CD69+ CD8+ T-cells in the spleen following immunization. Data represent mean ± SEM (n = 3–4). Left: ####p ≤ 0.0001, of the OVA + CAF04 group, as compared to unadj. OVA at 24 h, ****p ≤ 0.0001, of the OVA + CAF09 group, as compared to all other groups at 24 h. The gating strategies used for the flow cytometric analyses are shown in Supplementary data, Fig. S4. The data are also shown in Fig. S7, where the number of cells are presented.