Table 2.

Number of viable HK-2 cells (assessed by trypan blue exclusion assay), in different experimental conditions with or without co-stimulation recombinant CCL18 for 48 h

Condition with 0 ng/ml or 20 ng/ml of recombinant cytokine stimulation	Number of live cells for HK-2 cells stimulated without or with rCCL18 (x1000 cells per well, Mean ± SD)
M0	288 ± 17 **
H0	467 ± 22
M20	420 ± 22
H20	437 ± 43

Abbreviations: M0 = mannitol control + normal glucose, H0 = high glucose, M20 = mannitol + normal glucose + 20 ng/ml CCL18, H20 = high glucose + 20 ng/ml CCL18. The number of viable cells were assessed by direct cell count with trypan blue exclusion assay. The results are presented as mean ± SD. The data were tested by Analysis of Variance with Bonferroni’s correction for multiple comparisons. The number of viable cells were lower in cells culture in the M0 group (**p < 0.01), in comparison to the cells culture in other conditions. There was no significant differences in cell counts between H0, M20 and H20 groups