Fig. 1.

Protective effect of 3-O-methyl quercetin and kaempferol isolated from S. anacardium against H₂O₂ induced cytotoxicity. Proposed structures of isolated 3-O-methyl quercetin (a) and kaempferol (b). The Cytotoxic effect of H₂O₂ on the viability of the lung (L132) and liver (L02) cells at concentrations ranging from 20 to 200 μM for 24 h and results expressed as percent control (c). The Protective effect of isolated 3-O-methyl quercetin (S3) and kaempferol (S4) on H₂O₂ induced cytotoxicity in lung (d) and liver (e) cells. Cells were pretreated with isolated 3-O-methyl quercetin and kaempferol at concentrations ranging from 10 to 500 μg followed by 100 μM H₂O₂. The cytotoxic effect was determined by MTT assay and the results were expressed in-terms of viability as percent control. Each value represents mean ± SE of three independent experiments (n = 3). The values were significant at p < 0.05