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. 2016 Sep 29;13:45. doi: 10.1186/s12983-016-0177-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Localization of serotonin-like immunoreactivity, acetylated α-tubulin, and DAPI in Pyrosomella verticillata tetrazooid colony. a Adult blastozooids (b1 and b2), overview. Oral siphons (os) and cerebral ganglia (cg) highlighted. b Mature blastozooid highlighting oral siphon (os), pericoronal bands (pb), ciliated funnel (cf), gills (g), peribranchial tube (pt) and with motor nerves (anterior (an), lateral (ln), posterior (pn) and medial posterior (mpn) nerves) extending from the cerebral ganglion (cg). c Detail of the ciliated funnel (cf) and cerebral ganglion (cg) in dorsal view. d, e Light (d) and confocal (e) magnification of the cerebral ganglion (cg) (lateral view) in connection with the ciliated funnel (cf). f Detail of mpn crossing a peribranchial tube (pt). g Detail of the endostyle (serotonin-like immunopositive cells marked with arrowheads), with grayscale invert editing to highlight serotonin-like immunoreactive cell shape (inset). h Detail of the posterior part of one adult zooid, highlighting the pyloric gland (pg), with grayscale invert editing to highlight serotonin-like immunoreactive cell shape (inset)