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. 2016 Sep 29;9:101. doi: 10.1186/s13045-016-0329-3

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Purified γδ T cell stimulation with IL-15 and IPP for 5 days induces their proliferation. a Isolated γδ T cells were stimulated with IL-2+IPP (gray bar) or IL-15+IPP (dark bar) for 5 days. Unstimulated γδ T cells (white bar) were used as negative control. The percentage of proliferated (CFSE-diluted) cells within the viable γδ T cell population was determined by flow cytometry (n = 5) b. Histogram overlays show CFSE dilution and gating of unstimulated γδ T cells (gray-filled area) and γδ T cells exposed to IL-2+IPP or IL-15+IPP (black line) for one representative donor. Friedman test with Dunn’s multiple comparison test. **p < 0.01; ns not significant