Figure 5.

Primary urinary renal epithelial cells (URECs) from a healthy subject (WT) and a patient carrying a heterozygous mutation of P256S in HNF1B gene. (A) Mitotic localization of endogenous HNF1β (green) in heterozygous (P256S) or WT URECs at 37°C. (B) Mitotic localization of endogenous HNF1β (green) in heterozygous P256S or WT URECs at low temperature (25°C). Scale bar, 10 μm. (C and D) Quantification of the ‘ICQ’ between DAPI and GFP (HNF1β) signals at 37°C (panel C) or at 25°C (panel D), in mitotic URECs. Statistical significance between WT (n = 35) and P256S (n = 24) at 37°C, and between WT (n = 14) and P256S (n = 16) at 25°C was determined by Kruskal–Wallis test followed by Dunn's multiple comparisons test. *P < 0.05.