Figure 8.

Importazole affects the dynamic relocalization of P256S mutant on mitotic chromatin. (A) Representative images of MDCK cells expressing the P256S mutant stained with Hoechst (red signal, DNA) submitted to a cold shock, in the presence or absence of importazole. (B) Quantification of the difference of the Intensity Correlation Quotient (ΔICQ) between Hoechst and GFP signal before and after cold shock, in cells treated or not with importazole. Images after cold-shock were taken 1 min after addition of cold medium. White arrows indicate mitotic cells. Scale bars: 10 μm. Results are represented by mean ± SEM. Statistical significance between ΔICQ for DMSO (n = 27) and Importazole (n = 30) treated cells was determined by a paired t-test. **P < 0.001.