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. 2016 Jun 2;44(17):8179–8188. doi: 10.1093/nar/gkw509

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — (A) Coomassie-stained SDS gel of protein complexes used in this study. Subunit identities are as marked. Western blot analysis for Ies2 is shown below and the band is situated as indicated by the arrow. (B) SEC-MALS analysis of hIno80 core complex. The expected mass of a complex containing one subunit each of actin, Arp4, Arp5, Arp8, Ies2, Ies6 and affinity tagged Ino80 together with three copies each of Tip49a and Tip49b is 743 583 Da. The molecular weight determined by SEC-MALS is 802 000 ± 10 400 Da.