Table 2. ATPase data.
| A | |||
| Complex | Basal ATPase Rate (ATP/complex/min) | Basal ATP KM (μM) | |
| WT | 1.7 ± 0.1 | 323 ± 55 | |
| ΔIes2 | 2.8 ± 0.1 | 273 ± 36 | |
| ΔArp5, ΔIes6 | 2.4 ± 0.2 | 225 ± 63 | |
| ΔArp5, ΔIes6, ΔIes2 | 1.2 ± 0.2 | 244 ± 116 | |
| Arp5 [BP] | 1.0 ± 0.1 | 171 ± 55 | |
| Arp5 [BP], ΔIes2 | 7.5 ± 0.4 | 326 ± 44 | |
| Basal (non-nucleosome stimulated) ATPase kinetic data for the various Ino80 complexes. | |||
| B | |||
| Complex | Stimulated ATPase Rate (ATP/complex/min) | Stimulated NCP KM (nM) | ATPase Stimulation Factor |
| WT | 97 ± 3 | 9 ± 2 | 57 |
| ΔIes2 | 9.7 ± 0.7 | 47 ± 11 | 3 |
| ΔArp5, ΔIes6 | 83 ± 3 | 39 ± 5 | 35 |
| ΔArp5, ΔIes6, ΔIes2 | 4.9 ± 0.3 | 25 ± 7 | 4 |
| Arp5 [BP] | 200 ± 5 | 8 ± 1 | 200 |
| Arp5 [BP], ΔIes2 | 53 ± 2 | 12 ± 2 | 7 |
Nucleosome stimulated ATPase kinetic data for the various Ino80 complexes. These experiments were performed in the presence of 1 mM ATP over a range of nucleosome concentrations. Stimulation factor refers to the ratio of the stimulated to basal ATPase rates.