Skip to main content
. 2016 Aug 9;44(17):8335–8351. doi: 10.1093/nar/gkw702

Figure 1.

Figure 1.

Purification of Gemin5 associated factors in HEK293 cells. (A) Schematic of TAP-tagged Gemin5 polypeptides. Numbers indicate the amino acid residues flanking each polypeptide. Grey-pink ovals depict the WD40 motifs located within the N-terminal domain; a striped rectangle depicts a non-canonical RNA-binding site (RBS) located at the C-terminal domain. (B) Expression of the TAP-tagged G5-Nter, G5-Cter and the control TAP polypeptide in total cell extracts of transfected HEK293 cells monitored by WB using anti-CBP recognizing the TAP polypeptide. (C) Analysis of the TAP complexes by SDS-PAGE and silver staining. The size (kDa) of the MW markers is indicated on the left. An asterisk depicts the mobility of the G5-Ner and G5-Cter. (D) Western blot of total cell extracts (lysate) and TAP complexes (TAP-purification) associated to the TAP, G5-Cter or G5-Nter polypeptides. The small TAP polypeptide resulting from the TEV-digested control TAP sample was lost in this gel. A lower mobility of the G5-Cter TAP-purified sample results from TEV cleavage.