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. 2016 Aug 9;44(17):8335–8351. doi: 10.1093/nar/gkw702

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Gemin5 associates with the ribosomal particles in cell lysates. (A) HEK293 fractions corresponding to S30 and S100 (100 μg of total protein), ribosomes (R) and pure ribosomes (ribosome salt wash, RSW) (30 μg of total protein) were analyzed by Western blot to detect the presence of Gemin5, the translation initiation factor eIF3b and the ribosomal protein P0. (B) Polysome profile from a total lysate of HEK293 cells separated on a 10–50% sucrose gradient. The fractions corresponding to the supernatant (SN), 40S and 60S ribosomal subunits, the 80S ribosomes and the polysomes are indicated. Gemin5, eIF3b and the ribosomal protein P0 were analyzed in the fractions (15 μl) of the gradient by WB, using specific antibodies.