Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 9;44(17):8335–8351. doi: 10.1093/nar/gkw702

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 5. — Pull-down assays reveal direct interaction of Gemin5 with the ribosomal proteins L3 and L4. (A) GST-pull-down assay of ribosomal proteins L3 and L4, resistant to RNase treatment during the TAP procedure, with myc-Gemin5. GST protein alone was used as negative control. Gemin5 was immunodetected by WB using anti-G5; the GST-fusion proteins were detected using anti-GST. I depicts input; P, pull-down. Proteins L5, P0, RACK1 and S3A were used as negative controls. (B) GST-pull-down assay of ribosomal proteins L3 and L4 with the indicated mutants of His-G5_1–739. His-G5_1–739 mutants were immunodetected with anti-His, and the GST-fusion proteins were detected using anti-GST. GST protein alone was used as negative control. (C) GST-pull-down assay of RBPs, resistant to RNase treatment during the TAP procedure, with myc-Gemin5. Independent pull-down assays were conducted at least three times.