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. 2016 Aug 9;44(17):8335–8351. doi: 10.1093/nar/gkw702

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Gemin5 siRNA depletion decreased the polysome/80S ratio, and enhanced global protein synthesis. (A) Polysome profiles (10–50% sucrose gradient) from cytoplasmic lysates of siRNA Gemin5 depleted cells (red line) and siRNA control cells (blue line). Gemin5 level in the lysates (siRNAG5) was determined by WB with anti-Gemin5 by comparison to HEK293 cells (siRNAcontrol). Tubulin was used as loading control. (B) Representation of the polysome/80S ratio (mean ± SD) obtained from three independent polysome profiles of Gemin5-depleted or control cells. (C) Representation of the intensity values of [³⁵S]-met labeled proteins during a 3 h pulse in Gemin5 depleted and control cell extracts; values represent the mean ± SD obtained in three independent assays. Asterisks denote P-values (* P < 0.1; ** P < 0.05, *** P < 0.01).