Table 1. Influence on chromosome loss rate of conditional over-expression of genes identified through the prescreen.
| Prescreened | μcolony | Prescreened | μcolony | Prescreened | μcolony | Prescreened | μcolony |
|---|---|---|---|---|---|---|---|
| Gene | (n colonies) | Gene | (n colonies) | Gene | (n colonies) | Gene | (n colonies) |
| Assay 1 | Assay 3 | Assay 6 | Assay 9 | ||||
| MOT2 | 0.28 * (769) | MCM3 | 0.80 * (290) | CDC4 | 0.25 * (318) | CLN1 | 0.73 * (41) |
| NRM1 | 0.26 * (828) | CDC5 | 0.47 * (104) | YDR387C | 0.07 * (2708) | GRX2 | 0.52 * (61) |
| CDC20 | 0.21 * (800) | CLB5 (+) | 0.34 * (230) | CLN1 | 0.07 * (1344) | NOP6 | 0.42 * (77) |
| CLB5 (+) | 0.09 * (1033) | IME2 | 0.19 * (148) | BMT5 | 0.06 * (455) | SPC19 | 0.34 * (96) |
| YLR053C | 0.08 * (1132) | SLD3 | 0.09 * (182) | HMLα2 | 0.05 * (3637) | YRB1 (+) | 0.32 * (275) |
| SPO7 | 0.07 * (1791) | CWC24 | 0.05 * (946) | SLM4 | 0.05 * (274) | GPG1 | 0.28 * (79) |
| CDC6 | 0.05 * (844) | YIL025C | 0.02 (284) | YGL182C | 0.05 * (1616) | YFL051C | 0.18 (60) |
| CDH1 | 0.03 * (1429) | SMC2 | 0.02 (711) | YOS1 | 0.02 (996) | GSP2 | 0.16 (95) |
| MIX23 | 0.03 (1184) | PAN6 | 0.01 (650) | YAL069W | 0.02 (1792) | DAS1 | 0.14 (132) |
| YHM2 | 0.02 (624) | BMH2 | 0.01 (564) | MOT3 | 0.02 (863) | GSH2 | 0.10 (111) |
| BFR1 | 0.02 (612) | CLB2 | 0.01 (107) | RPS24B | 0.02 (1020) | Vector (-) | 0.05 (477) |
| ARP9 | 0.02 (1108) | PRP16 | 0.01 (1242) | AIM26 | 0.02 (1640) | Assay 10 | |
| RRT6 | 0.02 (1508) | TTI2 | 0.01 (264) | PGD1 | 0.02 (299) | YRB1 (+) | 0.66 (6) |
| YDR476C | 0.02 (881) | SUA7 | 0.01 (937) | CLN2 | 0.01 (1563) | COQ10 | 0.00 (35) |
| PGA1 | 0.02 (367) | Vector (-) | 0.01 (934) | Vector (-) | 0.01 (2876) | Vector (-) | 0.00 (14) |
| TTI2 | 0.02 (1552) | YKL202W | 0.01 (394) | SYS1 | 0.01 (941) | Assay 11 | |
| Vector (-) | 0.01 (3760) | VPS9 | 0.00 (402) | CKA2 | 0.01 (288) | YRB1 (+) | 0.28 * (157) |
| YNL198C | 0.01 (1088) | WSC2 | 0.00 (813) | RPL16A | 0.01 (2898) | ERP4 | 0.05 (775) |
| CDC40 | 0.01 (1937) | PTR2 | 0.00 (567) | PCL9 | 0.00 (2100) | Q0130 | 0.04 (749) |
| MBB1 | 0.01 (2508) | MSB3 | 0.00 (609) | CLB5 (+) | 0.00 (3557) | YPL071C | 0.04 (678) |
| FAD1 | 0.01 (1286) | TRP1 | 0.00 (969) | Assay 7 | Vector (-) | 0.04 (139) | |
| SWT1 | 0.00 (2516) | Assay 4 | YRB1 (+) | 0.84 (6) | YNL226W | 0.03 (614) | |
| SRB2 | 0.00 (4916) | NDT80 | 0.79 * (498) | RPL20A | 0.08 (867) | ADY4 | 0.02 (457) |
| Assay 2 | CLB5 (+) | 0.36 * (157) | ORC1 | 0.05 (840) | Assay 12 | ||
| XRS2 | 0.54 * (66) | REC8 | 0.35 * (838) | SRP14 | 0.03 (670) | SPC19 | 0.41 * (173) |
| YRB1 (+) | 0.39 * (57) | Vector (-) | 0.07 (475) | RHO4 | 0.01 (619) | GRX2 | 0.39 * (230) |
| HFI1 | 0.06 * (842) | Assay 5 | Vector (-) | 0.00 (5) | CLN1 | 0.28 * (68) | |
| MSM1 | 0.04 (289) | YRB1 (+) | 0.60 * (58) | Assay 8 | Vector (-) | 0.00 (149) | |
| SYC1 | 0.03 (383) | ELG1 | 0.33 * (317) | YRB1 (+) | 0.78 * (82) | ||
| JID1 | 0.02 (321) | DCG1 | 0.01 (289) | POL32 | 0.08 (543) | ||
| YER152C | 0.02 (215) | Vector (-) | 0.00 (55) | Vector (-) | 0.02 (142) | ||
| Vector (-) | 0.02 (355) | YJL077W-B | 0.02 (596) | ||||
| YGL082W | 0.01 (539) | RET3 | 0.01 (450) | ||||
| TFB5 | 0.01 (375) |
*These chromosomal loss rates per colony (μcolony) are significantly different from that of the isogenic strain harboring the empty MORF vector, under identical conditions in the same experimental set (Z-test, Bonferroni-adjusted P < .01). The Z-test was used to compare the chromosomal loss rates based on the Poisson parameters estimated from a Poisson regression. See Materials and Methods.