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Primers used for mutant and complement strain construction
| Primer | Sequencea (5′-3′) |
|---|---|
| PrkA-US | CGGAATTCTCCTTCGTTCGCGGA |
| PrkA-UA | CGGGATCCGACCTCGTTGCCGA |
| PrkA-DS | CGGGATCCGCCTTCCCATATCAG |
| PrkA-DA | GCTCTAGACACTTCCTCCGTGTCGT |
| CPA | ATGAAGTCGCTTAATCCCCCGGGCATCGGGTCCGGCGCCGGATCGGG |
| CPB | TAACAAAATATTAACGCCCCGGGTCAGCCCGCCTTGTTGACGCGCATG |
aRestriction enzyme sites are underlined
