Fig. 1.

Early and late effects of partial hepatectomy (PH) in Trim24 ^L2/L2Alb-Cre mice. a, b, c Early effects of PH in Trim24 ^L2/L2Alb-Cre mice versus wild type littermates. a Relative liver mass showed impaired and delayed liver regeneration, persistent during at least 15 days in Trim24 ^L2/L2Alb-Cre groups. Relative liver mass was obtained as following: mean liver mass of mice at the age of 2 months was measured in the first group (5 mice Trim24 ^L2/L2Alb-Cre and five mice wild type). In the groups sacrificed respectively at 48 h, 72 h, 5 and 15 days, the harvested livers were weighed and the relative liver mass was calculated as a percentage of the mean liver mass at the age of 2 months. b Ki67 labeling showed significantly lower proliferation rates at 48 and 72 h after PH with abnormal prolonged proliferation at 15 days after surgery in Trim24 ^L2/L2Alb-Cre mice. c Apoptosis level (TUNEL labeling) demonstrated a significantly higher level of apoptosis in Trim24 ^L2/L2Alb-Cre mice, not influenced by the realization of PH. d, e, f Late effect of PH: histological findings (HE staining, original magnification x240) in the livers of Trim24 ^L2/L2Alb-Cre mice at 5 months after PH. d Clear cell foci (dysplastic hepatocytes, with preserved lobular structure of the liver and no compression) in 5 out of 6 Trim24 ^L2/L2Alb-Cre mice. e Hepatic adenoma in 3 out of 6 Trim24 ^L2/L2Alb-Cre mice, with no portal structure within the affected area and compression of neighbouring hepatocyte plates (black arrow heads). f Invasive hepatocarcinoma in 1 out of 6 Trim24 ^L2/L2Alb-Cre mice, with loss of portal structures, carcinoma infiltration of neighbouring hepatocyte plates (black arrowheads) and numerous mitotic figures as hallmark of malignant lesion (white arrowheads)