Figure 3. RAGE expression in parental and RAGE-transfected HT-1080 cells, and effect of collagen aging on cell proliferation.

Parental HT-1080 and HT-1080 cells stably transfected with empty vector (HT-1080 ^Neo), human full length RAGE (HT-1080 ^{Full RAGE}) and dominant negative RAGE (HT-1080 ^{DN RAGE}) were cultured on plastic. A. RAGE transcripts contents in each cell lines were evaluated by real-time quantitative polymerase chain reaction. B. RAGE protein expression was analyzed by western blot. Glyceraldehyde 3-phosphate deshydrogenase (GAPDH) was used as a loading control. C. Parental and transfected HT-1080 cells were seeded in adult and old type I collagen 3D matrices at a density of 1.5 × 10⁴ cells/ml. After 5 days of culture, cell density was evaluated by phase contrast microscopy. Data shown are representative of three independent experiments (N.S. = not significant).