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. 2016 Apr 18;7(18):24908–24927. doi: 10.18632/oncotarget.8795

Figure 4. Effect of β1 integrin inhibition on HT-1080 cell proliferation.

Figure 4

A. HT-1080 cells were transfected with control siRNA (Ctrl) or β1 integrin siRNA. β1 integrin expression was analyzed by RT-PCR. Actin was used as a control. HT-1080 cells were seeded in adult and old type I collagen 3D matrices at a density of 5 × 104 cells/ml, with or without B. siRNA directed against β1 integrin, or C. blocking antibody against β1 integrin (10 μg/ml). After 5 days of culture, cell density was evaluated by phase contrast microscopy. Values represent the mean ± S.E.M. of three independent experiments (N.S. = not significant).