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. 2016 Mar 22;7(18):25224–25240. doi: 10.18632/oncotarget.8279

Figure 2. ACL Ser-455 phosphorylation is widely elevated in breast cancer.

Figure 2

A. Tissue array with normal breast (n = 8), hyperplasia (n = 10), invasive ductal (n = 18) and invasive lobular (n = 8) tissue samples were subjected to IHC with a phospho-ACL antibody as described in Methods. Representative images are shown (left) and staining results are summarized (right). B. Breast tumor cell lines were grown in 10-cm culture dish. Exponentially proliferating cells were fed for 24 h with medium with or without 10% serum. Total cell lysates were subjected to immunoblotting. C. MDA453 cells were treated with the indicated inhibitors followed by immunoblotting. D. Rat2 and Rat2-myr-AKT cells were treated inhibitors for 24 h followed by immuoblotting.