Figure 1. DNA damage response analysis in DT40 cells and their DNA repair mutants exposed to H₂O₂.

Cells were exposed to different concentrations of freshly prepared H₂O₂ at 37°C. Survival data were log-transformed giving approximate normality. Analysis of covariance (ANCOVA) was used to test for mean intercept differences and differences in the slopes of the linear dose response curves between wild type and a series of mutant cells. Error bars indicate the 95% confidence intervals. HR- homologous recombination; NHEJ- Non-homologous end joining; BER/ NER- Base excision repair/ Nucleotide excision repair; MMR- Mismatch repair; DDCP- DNA damage checkpoint; TLS- Translesion synthesis.