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. 2016 Mar 23;7(18):25443–25460. doi: 10.18632/oncotarget.8327

Figure 1. ERM expression and localization in SKBR3 breast cancer cells.

Figure 1

(A) Western blot analysis of ERM levels in SKBR3, HeLa and PC3 cells. SKBR3 breast cancer cells do not express moesin. (B) Colocalization of ezrin/radixin and ErbB2 in SKBR3 cells. 3D-SIM of fixed cells, stained for endogenous ERM and ErbB2, shows a high degree of colocalization between the ezrin/radixin and ErbB2 at the plasma membrane (left panel: max. projection; middle: single plane section; right: single channels of insert). Scale bars: 10 μm. (C) Analysis of protein association in SKBR3 cells by proximity ligation assay (PLA). 2 h treatment with 3 μM GA leads to decreased association of ezrin/ErbB2 and radixin/ErbB2. Data is represented as mean +/− SEM (***P < 0.001). (D) Corresponding single plan section of a representative PLA experiment. Fluorescence and DIC pictures of control cells (upper panel) and cell treated for 2 h with geldanamycin (lower panel) are shown. Scale bars: 10 μm.