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. 2016 Mar 25;7(18):25683–25697. doi: 10.18632/oncotarget.8361

Figure 2. miR-18a encapsulated in OGNVs inhibits liver metastasis of colon cancer and induces Kupffer cell polarization into M1.

Figure 2

Figure 2

(A) Schematic representation of the treatment schedule. All groups of mice were euthanized 14 days after the intra-splenic tumor inoculation, and tumor specimens were obtained for analysis. (B) Frequency of MHCII, TGFβ, IL-12, IFNγ, CD80, CD86, CD206, and IL-10 positive cells in liver F4/80+ cells from naive BALB/c mice, CT26 liver metastasis mice treated with OGNVs packing control miRNA (OGNVs/Ctrl) or OGNVs packing miR-18a (OGNVs/miR-18a) assessed by flow cytometry. (C) The histogram shows the quantification of results at (b). (D) Expression of mature miR-18a, MHCII, TGFβ, IL-12, IFNγ, and iNOS in liver F4/80+ cells was assessed by qPCR. (E) Representative livers (up) and representative hematoxylin and eosin (H & E)-stained sections of livers (middle, 20×; bottom, 400× magnification). (F) Liver weight (left) and liver metastatic nodule number and size (right). (G) Survival of mice after intra-splenic injection of CT26 cells. (H) Frequency of IFNγ+ cells in liver CD3+Dx5+ (NKT) cells, CD3−Dx5+ (NK) cells, and CD3+ Dx5− (T) cells. Right, quantification of results; each symbol represents an individual mouse. *P < 0.05 (two-tailed t-test). Data are representative of three independent experiments (error bars, S.E.M.).