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. 2016 Mar 25;7(18):25698–25711. doi: 10.18632/oncotarget.8365

Figure 2. Cancer stem cells express high level of GAS6 in PCa cells in bone marrow microenvironment.

Figure 2

(A) Experimental model of isolation of PCa CSC cells in vitro cocultures of PCa cells with osteoblasts (OB). (B, C) Expression of GAS6 mRNA in CD133–/CD44– or CD133+/CD44+ populations from the cocultures of PCa cells with osteoblasts in vitro as quantified by real-time PCR. Data are representative of mean with s.d. (Student's t-test). (D) Experimental model of isolation of PCa CSC cells in marrow in vivo. (E, F) Expression of GAS6 mRNA in CD133–/CD44– or CD133+/CD44+ populations from DTCs in bone marrow at 24 hours after i.c. injection of PCa cells in SCID mice as quantified by real-time PCR. Data are representative of mean with s.d. (Student's t-test). (G) Immunofluorescence staining of CD133 (red)/GAS6 (green), CD44 (red)/GAS6 (green), or CD133 (red)/CD44 (green) (white arrows) in PCa cells in bone marrow of a PCa patient. Blue, DAPI nuclear stain. Bar = 50 μm.