Figure 4. GAS6 overexpression activates the phosphorylation of Mer signaling in prostate epithelial cells or cancer cells.

(A) GAS6 mRNA expression as quantified by by real-time PCR in PCa^Control cells or PCa^GAS6OE cells. (B) GAS6 protein as quantified by ELISA in PCa^Control cells or PCa^GAS6OE cells. Data in Figure 4A, B are representative of mean with s.d. (Student's t-test). (C, D). PCa^Control cells or PCa^GAS6OE cells were starved for 24 hours and then exposed to 10% FBS of culture condition for 1 hour and phosphorylation of Mer was evaluated by (C) Western blot or (D) immunofluorescence staining. Bar = 50 μm. (E, F) GAS6 and Mer mRNA expression in the PrEP^GAS6+/+ cells or PrEP^GAS6−/− cells by real-time PCR. (G) Mer (red)/GAS6 (green) or p-Mer (red)/GAS6 (green) in the PrEP^GAS6+/+ cells or PrEP^GAS6−/− cells was evaluated by immunofluorescence staining. Bar = 50 μm.