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A. The protein expression of β-catenin and its target gene c-myc was analyzed using western blot assay. β-actin served as loading control. B. The expression of β-catenin (green) in L1, L2, and SW620 was analyzed using confocal microscopy. DAPI (blue) was used for nuclear stain. C. and D. Western blot to determine the effect of β-catenin or E-cadherin knockdown on EMT marker expression in L1 and L2. β-actin served as loading control.
