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. 2016 Mar 23;7(18):25971–25982. doi: 10.18632/oncotarget.8300

Figure 3. Knocking down COX-2 inhibites SUV-induced the phosphorylation of p38 or JNKs in HaCaT cells.

Figure 3

A. Expression of COX-2 in HaCaT cells was analyzed by Western blot. HaCaT cells were transfected with shMock or shCOX-2 (#1-#5), cell lysates were analyzed by western blot. B. Production of PGE2 in shMock or shCOX-2 (#1-#5) cells were measured by ELISA as described in Materials and Methods. COX-2 was dramatically decreased in shCOX-2 #1 and #3 cells, which were selected for further experiments. Data are presented as means ± SEM (n=3), *P < 0.05, ***P < 0.001, representing significant decrease compared with shMock cells. C. Knockdown of COX-2 inhibited SUV-induced phosphorylation of p38 or JNKs. HaCaT shMock cells and shCOX-2 #1 and #3 cells were stimulated with SUV (40 KJ/m2) and then harvested at 5 min. Proteins were analyzed by western blot. Data were representative of triplicate experiments.