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. 2016 Mar 23;7(18):25971–25982. doi: 10.18632/oncotarget.8300

Figure 4. Salidroside can directly bind with COX-2 and has no toxicity to HaCaT and JB6 Cl41 cells.

Figure 4

A. The chemical structure of salidroside. B. Proposed molecular model of salidroside binding with COX-2. Salidroside binds to the active site of COX-2 by forming hydrogen bonds with protein amino acid residues ARG106 and TYR341. C. A pull-down assay was performed to detect the binding of salidroside with COX-2 in vitro. Lane 1 was input control (COX-2 protein standard); Lane 2 was the negative control, indicating no binding between COX-2 and Sepharose 4B beads; lane 3 indicated that COX-2 binds with Salidroside-Sepharose 4B beads. D. Salidroside had no cytotoxity on HaCaT and JB6 Cl41 cells. The cell viability was determined by MTS assay according to Materials and Methods. Data are shown as means ± standard deviation from triplicate experiments.