Figure 5. Salidroside inhibits the activity of COX-2, decreases the activation of p38 or JNKs signaling pathway and production of inflammatory factors in SUV-irradiated cells.

A. Salidroside inhibited the expression of COX-2 in a time-dependent manner, the obvious inhibition was observed at 12 h after SUV exposure. The cell lysates (30 μg) were subjected to 10% SDS-PAGE gel. B. Salidroside inhibited COX-2 activity and then markedly reduced PGE₂ production in HaCaT and JB6 Cl41 cells. The PGE₂ released in medium was measured by PGE₂ assay kit according to the instructions described in Materials and Methods. C and D. Phosphorylation of p38 or JNKs was substantially attenuated in a dose- and time-dependent manner after SUV irradiation with salidroside treatment in HaCaT and JB6 Cl41 cells. Cells were pre-treated with salidroside and stimulated with SUV as indicated. The whole cell lysates were analyzed by western blot. E. Salidroside inhibited the secretion of IL-6 and TNF-α induced by SUV. Significant differences compared with the group treated with SUV alone (*P<0.05; **P<0.01; ***P<0.001).