Figure 2. Combined treatment reduced cell viability and motility more than monotherapies.

A. The effect of Huaier extract and TAM on cell viability was measured by MTT assay as described in Materials and Methods. Combined treatment inhibited viability in both cell lines in a dose- and time-dependent manner. MCF-7 B. and T47D C. cells formed colonies. Representative Giemsa staining pictures of the colonies are shown. MCF-7 and T47D cell colonies D. were counted. Cell mobility was strongly inhibited by combined treatment as shown by migration E. and invasion F. assays using the Transwell system. Giemsa was used as a staining agent and cell numbers were counted in six representative fields. Bars, 50 μm. All experiments were performed in triplicate and data are presented as the mean ± SD of three separate experiments (*p < 0.05; **p < 0.01; ***p < 0.001 vs. the control group).