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. 2016 Mar 23;7(18):26003–26015. doi: 10.18632/oncotarget.8303

Figure 3. Huaier extract synergizes with tamoxifen to induce autophagy in ER-positive breast cancer cells.

Figure 3

MCF-7 A. and T47D C. cells were labeled with Acridine orange (AO) after the indicated treatments and quantified using flow cytometry. FL1-H indicates green color intensity (cytoplasm and nucleus), whereas FL3-H shows red color intensity (AVO). Cells in up quadrants were considered AVO-positive. Treated MCF-7 B. and T47D D. cells were stained with AO and examined under a fluorescence microscope. Bars, 50 μm. All of the experiments were performed in triplicate and data are presented as the mean ± SD of three separate experiments (*p < 0.05; **p < 0.01; ***p < 0.001 vs. the control group).