Figure 4. Huaier extract synergizes with tamoxifen to induce apoptosis in ER-positive breast cancer cells.
Flow cytometric analysis of PI-Annexin-V to quantify apoptosis in MCF-7 A. and T47D C. cells after treatment as described in Materials and Methods. Representative TUNEL staining (red fluorescence) of MCF-7 B. and T47D D. cells treated with Huaier (0 or 4mg/ml) and TAM (0 or 10μmol/L). Bars, 50 μm. Columns are the average of three independent experiments. (*p < 0.05; **p < 0.01; ***p < 0.001 vs. the control group).