Figure 7. Combined treatment enhanced the suppression of tumorigenesis in a xenograft model.
A. Flank tumors were established in BALB/c nu/nu female mice as described in Materials and Methods. Mice were killed 40 days after flank injection; representative tumors are shown. B. Growth curves of xenograft tumors after the injection of MCF-7 cells. Mean tumor volumes ± SD are shown. C. Immunohistochemical analysis of Bcl-2(C), Ki67 D. and Lc3b E. from the collected xenografts. Bars, 50 μm. F. Combined drug treatment increased levels of Bax, Beclin-1, and Lc3b and decreased Bcl-2 and Cyclin D1 levels compared to the control and monotherapy groups. The experiments were performed in triplicate and data are presented as the mean ± SD of three separate experiments (*p < 0.05; **p < 0.01; ***p < 0.001 vs. the control group).