Figure 2. Isoflurane, Sevoflurane and Desflurane alter mRNA expression levels of tumour metastasis genes shown by RT-PCR.

SK-OV3 cells were treated with air (N₂) or 2% isoflurane (Iso) or 3.6% sevoflurane (Sevo) or 10.3% desflurane (Des) for 2 hours, and then recovered in the normal cell incubator for up to 24 hours. Six hours after exposure analysis of the tumour metastasis PCR array was carried out. Results obtained from the tumour metastasis PCR array and RT-PCR analysis are well correlated. All data is displayed as relative to the endogenous control, β-Actin (n = 4). Data are expressed as mean ± SD. NC: naïve control. Iso: isoflurane, Sevo: sevoflurane, Des: desflurane.