Skip to main content
. 2016 Mar 25;7(18):26220–26234. doi: 10.18632/oncotarget.8354

Figure 2. Effect of TCF21 on the transcriptional activity of ERα.

Figure 2

A–B. MCF-7 and ZR-75-30 cells were transfected with ERE-luciferase and Flag-TCF21. Luciferase activity was detected either with or without pre-treatment of the cells with 10 nM E2 for 16 h. For comparison, the ERE-luc activity level of control cells was set to 1. ‘*’ and ‘**’ indicate significantly different from cells transfected with ERE-luc only at the P<0.05 and P<0.01 level, respectively. C–D. MCF-7 and ZR-75-30 cells were transfected with Cyclin D1-luciferase and shCN, shTCF21-1 or shTCF21-2. Luciferase activity was detected with or without pre-treatment of the cells with 10 nM E2 for 16 h. For comparison, the Cyclin D1-luc activity level of control cells was set to 1. ‘*’ indicates significantly different from cells transfected with Cyclin D1-luc and shCN at the P<0.05 level. E. MCF-7 cells were transfected with ERE-luc and Flag-TCF21. Luciferase activity was detected either with or without 10 nM TSA for 20 h. For comparison, the ERE-luc activity level of control cells was set to 1. ‘*’ indicates significantly different from cells treated with TSA at the P<0.05 level. F. MCF-7 cells were transfected with shTCF21-1. Cells pre-treated with or without 10 nM E2 for 16 h were subjected to PCR to measure the mRNA levels of pS2 and Cyclin D1. G. MCF-7 cells transfected with shTCF21-1 were treated with or without 10 nM E2 for 16 h. The samples were subjected to Western blot analysis with the indicated antibodies. H. ChIP experiment showing the binding of TCF21 and ERα to ERE of the pS2 promoter in MCF-7 cells. MCF-7 cells transfected with shERα were treated with or without 10 nM E2 for 16 h. The cells were subjected to IP with anti-IgG, anti-ERα, or anti-TCF21 antibody. All experiments were repeated at least three times. Data are the means ± SDs of three independent experiments.