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. 2016 Mar 25;7(18):26220–26234. doi: 10.18632/oncotarget.8354

Figure 3. Sumoylation of TCF21.

Figure 3

A. Schematic representation of the primary structures of TCF21 and the predicted sumoylation sites along with the structures of TCF21 in different species. B. HEK 293T cells transfected with HA-TCF21 and Flag-SUMO1, 2 or 3 were subjected to Western blot with anti-HA antibody. C. HEK 293T cells co-transfected with GFP-SUMO1 or GFP-SUMO1 mutant and HA-TCF21 were subjected to Western blot with anti-HA antibody. D. HEK 293T cells were subjected to IP using anti-TCF21 antibody or anti-IgG antibody followed by Western blot using anti-SUMO1 or anti-TCF21 antibody. E. MCF-7 cells treated with or without E2 were subjected to IP with anti-TCF21 antibody followed by Western blot with anti-SUMO1 antibody. F. HEK 293T cells were transfected with different combinations of constructs as indicated, followed by Western blot with anti-HA antibody. No NEM was added in the cell extracts. G. HEK 293T cells were transfected with Flag-tagged wild-type or mutant TCF21(K24R, K65R or 2KR) and GFP-SUMO1, and individual cell extracts were subjected to Western blot with anti-Flag antibody. All experiments were repeated at least three times.