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. 2016 Mar 25;7(18):26220–26234. doi: 10.18632/oncotarget.8354

Figure 5. Effect of TCF21 sumoylation on the transcriptional activity of ERα.

Figure 5

A–B. MCF-7 and ZR-75-30 cells were transfected with the indicated plasmids. Luciferase activity was detected either with or without pre-treatment of the cells with 10 nM E2 for 16 h. For comparison, the ERE-luc activity level of control cells was set to 1. ‘**’ indicates significantly different from cells transfected with ERE-luc and Flag-TCF21 at the P<0.01 level. ‘*’ indicates significantly different from cells transfected with ERE-luc and mutant TCF21 (K24R) at the P<0.05 level. ‘ns’ indicates not significant. C–D. MCF-7 and ZR-75-30 cells were transfected with Cyclin D1-luc, Flag-TCF21, Flag-TCF21 (K24R), or Flag-TCF21(D26A). Luciferase activity was detected either with or without pre-treatment of the cells with 10 nM E2 for 16 h. For comparison, the level of Cyclin D1-luc activity in control cells was set to 1. ‘**’ indicates significantly different from cells transfected with Cyclin D1-luc and Flag-TCF21 at the P<0.01 level. ‘*’ indicates significantly different from cells transfected with Cyclin D1-luc and mutant TCF21(K24R) at the P<0.05 level. ‘ns’ indicates not significant. E. MCF-7 cells were transfected with Flag-tagged wild-type TCF21 or mutant TCF21(K24R). Cells pre-treated with or without 10 nM E2 for 16 h were subjected to ChIP to examine the recruitment of ERα, TCF21, HDAC1/2 and acetyl-H3K27 to the pS2 promoter. All experiments were repeated at least three times. Each bar represents the mean ± SDs of three independent experiments.