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. 2016 Mar 28;7(18):26496–26515. doi: 10.18632/oncotarget.8420

Figure 2. RLR pathway mediates radiation-induced gastrointestinal death following total body irradiation.

Figure 2

A. Overall survival following total body irradiation (TBI, 5.5 Gy) of age-matched (9-12 weeks) wild-type (C57BL/6 or ICR background) and germline deleted LGP2−/− (left), RIG-I−/− (middle), and MDA5−/− (right) mice. Differences in survival were assessed using log-rank tests. *P < 0.05, **P < 0.01, n.s. – not significant. B. IFN-beta quantification in mouse serum at specified time-points following exposure to TBI (5.5 Gy). Horizontal bar denotes mean value. Error bars are SEM. C. Small intestinal TUNEL staining of C57BL/6 wild-type (WT) and LGP2−/− mice prior to and 7 days following total body irradiation at 5.5 Gy. Small intestinal cross-sections from LGP2−/− mice exhibited greater intestinal crypt destruction (denoted by red arrows) as well as increased apoptosis (brown staining) in the crypt cells and the enterocytes lining the microvilli as compared to wild-type mice. D. Small intestinal TUNEL staining of C57BL/6 wild-type (WT), ICR RIG-I+/+ WT and ICR RIG-I−/− mice prior to and 13 days following total body irradiation at 5.5 Gy. Small intestinal cross-sections from RIG-I−/− mice showed minimal apoptotic staining in the enterocytes lining the microvilli as compared to wild-type mice. All images are representative of three replicates per condition. Magnification, 20x; scale bars, 0.11 μm.