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. 2016 Mar 31;7(18):26765–26779. doi: 10.18632/oncotarget.8503

Figure 6. ATOH8 is epigenetically silenced in NPC cells induced by LMP1.

Figure 6

A. the promoter characteristics of ATOH8 according to UCSC genome browser. RRBS, MER-seq CpG and MeDIP CpG tracks present the methylation status of ATOH8 promoter in different cells. Layered H3K4me3, H3K4me3 and H3K27me3 tracks display chromatin states of ATOH8 promoter. MSP-1 and MSP-2 indicated methylation specific PCR (MSP) detection regions. ChIP-ctrl, ChIP-1, ChIP-2, ChIP-3 and ChIP-4 indicated ChIP Real-Time PCR detection regions. B. products of MSP with indicated regions in ATOH8 promoter in CNE1 cells were analysis with agarose gel electrophoresis. M, methylated template. U, unmethylated template. C. & D. products of ChIP using H3K4me3 or H3K27me3 antibody in CNE1 cells were subjected to PCR and Real-Time PCR analysis. The relative levels of ATOH8 promoter occupancy were examined by Real-Time PCR and calculated using ΔΔCt value. g-DNA indicated the primers work well when using genome DNA as template. *p < 0.05, **p < 0.01, ***p < 0.001, two-tailed Student's t-test.