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. 2016 Apr 8;7(18):26806–26822. doi: 10.18632/oncotarget.8645

Figure 3. Phenotypes of MMP10 knockdown cells.

Figure 3

(A) Sphere formation of MMP10 knockdown cells. Seven-day-cultured MMP10 knockdown cells of RMG1 (upper) and AMOC2 (lower) cell lines under × 100 magnification light microscopic view. Bar scales are 100 μm. (B) Sphere-forming assay of MMP10 knockdown cells. Data are shown as means ± SD. (C) ALDEFLUOR assay for MMP10 knockdown cell lines. (D) Expression of stem cell-related genes in MMP10 knockdown cells. Expression levels of stem cell-related genes were examined by quantitative PCR using the ΔΔCT method. Data are shown as means ± SD. (E) Resistance of mock and MMP10-overexpressed cells to chemotherapeutic. Calculated cell viability after 48 h of treatment with paclitaxel (PTX) and carboplatin (CBDCA) is shown in bar graphs. X-axis is dose intensity of each agent. Y-axis is calculated cell viability from the counted number of surviving cells. Data are shown as means ± SD. All statistical analyses for this Figure were performed using bilateral Student's t test. P-values are shown as follows: *< 0.05, **< 0.01.