Table 2.
Gene expression pattern analysis of regulated signal transduction pathways
| Signal transduction pathway associations | P value | Genes |
|---|---|---|
| Pathways upregulated in reactive astrocytes at 5 days after stab wound injury | ||
| NF kappa B | 6,54 E −06 | Cd14, Cd83, Aif1, Litaf, Nfkbia, Cebpb, Nfkbid, Cxcl1, Ier3, Cd74, Bcl3, Il1b, Tnfrsf1b, Spp1, Nfkbib, Bcl2a1a, S100a4, Ccl4, Siva1, Saa3, Malt1, Pycard |
| Tumor necrosis factor (TNF superfamily, member 2) | 2,77 E −05 | Cd14, Cd83, Aif1, Litaf, S100a6, Nfkbia, H2‐Ab1, Cxcl1, Ctsd, Il1b, Tnfrsf1b, Nfkbib, Ccl4, Saa3, Socs3, Erg |
| Spleen tyrosine kinase | 1,11 E −04 | Tyrobp, Sla, Lgals1, Fcgr1, Cmtm7, Ptprc, Fcer1g, Trem2, Egr2 |
| Caspase | 1,84 E −03 | Lgals1, Plp1, Ctsd, Afp, Ctsc, B2m, Siva1, Malt1, Pycard, Creg1 |
| Chemokine (CC MOTIF) ligand 2 | 2,52 E −03 | Cd14, Nfkbia, Cxcl1, Il1b, Vim, Ccl4 |
| Interleukin 10 | 3,07 E −03 | Cd83, Lgals1, Il1b, Etv3, Fcgr1, Trem2, Socs3 |
| Interleukin 1 | 3,95 E −03 | Cd14, Nfkbia, Cxcl1, Il1b, Nfkbib, Ctsc, Ptgds, Socs3, Pycard |
| Interleukin 6 | 4,21 E −03 | Grn, Cebpb, Cxcl1, Lgals3, Il1b, Gfap, Junb, Socs3 |
| Toll‐like receptor | 6,94 E −03 | Cd14, Cxcl1, Grk5, Anxa2, Hspe1, Ly86, Saa3, Trem2 |
| Protein tyrosine phosphatase, receptor type | 8,19 E −03 | Tyrobp, Lgals1, Ptpre, Fcgr1, Cd52, Ptprc |
| Pathways downregulated in reactive astrocytes at 5 days after stab wound injury | ||
| Fibroblast growth factor | 8,99 E −05 | Ccnd2, Syt1, Serpine2, Fgfr3, Pax6, Sdc4, Sox2, Nedd4, Gpc5, Dmp1, Ncam1, Vegfa, Gja1, Spry2, Hip1, Hs2st1, Gli3, Sox9, Etv4, Cyr61 |
| Rhoa RAS homolog | 4,78 E −04 | Neo1, Arhgap5, Ednrb, Rock1, Gna13, Pias1, Sdc4, Ophn1, S1pr1, Plxnb1, Rapgef3, Sox9, Sdc2, Cyr61 |
| BETA catenin | 1,75 E −03 | Faf1, Tle1, Fzd4, Ppap2b, Sfrp1, Fbxw11, Rora, Sox2, Chd8, Prdm5, Tcf7l2, Trrap, Enah, Ptn, Sox9, Sdc2, Apc, Cyr61, Zeb1, Ptprz1 |
| Lymphoid enhancer binding factor 1 (TCF/LEF) | 1,85 E −03 | Tle1, Ppap2b, Nrarp, Sfrp1, Tcf7l2, Hipk2 |
| Paxillin | 2,00 E −03 | Pld2, Gna13, Ptpn11, Adcyap1r1, Nek3, Flii, Ptprt, Hgf |
| Homeodomain interacting protein kinase | 4,01 E −03 | Daxx, Pax6, Tcf7l2, Zbtb4, Hipk2 |
| Wingless type | 4,44 E −03 | Faf1, Tnik, Tle1, Fzd4, Ppap2b, Fermt2, Znrf3, Nrarp, Lrp4, Sfrp1, Rora, Pax6, Sox2, Chd8, Prdm5, Tcf7l2, Rhou, Trps1, Trrap, Enah, Gli3, Sox9, Sdc2, Apc, Macf1 |
| Progestin and ADIPOQ receptor | 7,64 E −03 | Srebf1, Ppara, Paqr8, Appl2 |
| Nuclear receptor subfamily 2, group F, member 2 | 8,13 E −03 | Srebf1, Ppara, Paqr8, Appl2 |
| Pathways upregulated in both the reactive astrocytes at 5 days after stab wound injury and the adult NSCs | ||
| Aurora kinase | 3,03 E −03 | Cdc20, Ncaph, Vim, Ccna2, Cdk1 |
| Mixed lineage kinase | 3,22 E −03 | Pim1, Txn1, Gspt1, Nfkbia, Gadd45b, Anxa2 |
| Cyclin B1 | 3,61 E −03 | Cdc20, Pbk, Ccna2, Cdk1 |
| Cell division cycle 2, (G1 to S and G2 to M) | 4,14 E −03 | Cdc20, Pbk, Ncaph, Vim, Ccna2, Cdk1 |
| Minichromosome maintenance complex | 4,49 E −03 | Ppp2r3c, Ccna2, Cdk1 |
| Cell division cycle 25C | 4,78 E −03 | Pim1, Ccna2, Cdk1 |
| BUB1 mitotic checkpoint serine/threonine kinase | 5,64 E −03 | Cdc20, Cdk1 |
Shown are the significantly (P < 0.01) enriched signal transduction pathways identified using GePS for genes significantly (FDR < 10%) regulated in reactive relative to nonreactive astrocytes or upregulated (P < 0.01, fold change > ×2) in both the adult NSCs and reactive astrocytes in comparison to nonreactive astrocytes.