Fig. 6.

Host toxicity by haemolytic and tetrazolium-based assays. Human RBCs were treated for 1 h with WK12 or WR12 in PBS, and percentage haemolysis was determined by spectrophotometric analysis of released haemoglobin using a standard curve of RBC lysis (a). To determine the cytotoxicity against nucleated cells, human monocyte-derived macrophages were treated with peptides for 1 h and cytotoxicity was determined by MTT assay (b). Data plotted are mean values (in triplicate) from three independent experimental trials, and statistical analysis by multiple t-tests showed no significant differences between WR12 and WK12 for each test concentration (P > 0.2).