Fig. 5.

Transgenic mouse bioassay of CWD derived from experimentally infected sheep. Shown are the outcomes of intracerebral inoculation of Tg338 (a) or TgElk (b) mice with CWD prions derived for experimental infections of sheep CFIA113 and CFIA122. Closed circles, mice culled at the onset of clinical disease and PrP^res detected in the brain; open circles, preclinical mice (culled due to intercurrent health issues or during the endpoint period with no clinical signs, but PrP^res detected in the brain); open squares, mice with no detectable PrP^res in the brain at the time of cull. Censored data are included. All Tg338 mice inoculated with sheep brain homogenate harbouring CWD prions had detectable PrP^res in the brain when culled in the endpoint period. Some of the similarly inoculated TgElk mice were culled due to the onset of clinical disease just prior to the endpoint period.