Table 2. Transmission of CWD from white-tailed deer with naturally acquired clinical disease or experimentally inoculated sheep to ovinized (Tg338) and cervidized (TgElk) mice.
| Mouse | Inoculum* | Mice culled at onset of clinical disease† /total inoculated |
Median survival time (95 % CL‡) | Attack rate in mice§ |
|---|---|---|---|---|
| Tg338 | WTD804 | 0/10 | >389 (NE) | 0/10 |
| CFIA113 | 0/9 | >499 (NE) | 9 (6)/9 | |
| CFIA122 | 0/9 | >499 (NE) | 9 (3)/9 | |
| TgElk | WTD804 | 9 (1)/10 | 121 (113, 128) | 10/10 |
| CFIA113 | 2 (7)/12 | >290 (290, NE) | 10 (1)/12 | |
| CFIA122 | 5/10 | >261 (261, NE) | 6 (1)/10 |
*WTD, White-tailed deer; CFIA, experimental intracranial inoculation of sheep with pooled brain and lymph node homogenate from elk with CWD (see Methods for more detail).
†Number of mice culled at the onset of clinical disease/number of mice inoculated. The number in parenthesis indicates additional mice with suspect clinical signs that also had intercurrent health issues at the time of cull. These mice were censored for survival analysis.
‡95 % lower and upper confidence limits (LCL, UCL): In the case of right censored survival analysis, lower confidence limits are left bounded, whereas upper confidence limits are unbounded. NE, Non-estimable due to insufficient group sample size for which survival time could be measured.
§Number of mice with detectable protease resistant prion protein (PrPres) in the brain at the time of cull/number of mice tested. All mouse brains were tested by standard (direct lysis) Western blot (WB); brain tissue negative by standard WB was tested using PTA-WB. The number in parenthesis indicates brains PrPres positive by PTA-WB which is included in the total number positive.