Table 1.

Protein–ligand interactions measured by MS under non-denaturing conditions

Proteins (peptides)	Ligands	KD (μM)		References
		MS	Othersa
FK506 binding protein	FK506 (drug)		0.0004	[5]
Globin	Heme			[6]
Vancomycinb	Peptide	1–90	1–170	[13]
OppA	Peptide		56–2,900	[22]
Replication terminator protein	DNA	≤0.002	0.0005	[23]
Chorismate mutase	Inhibitor	1.7	1.0	[24]
RNase A	2′CMPc	2.0	1.0	[25]
hGHbpd	Compounde	0.76	0.7	[14]
Beta-peptide	Zinc	20		[26]
Antigen binding fragment	Hexasaccharide ligand	6.3	5.9	[27]
Norovirus P domain	HBGA oligosaccharide	333–2,778		[28]
ABC transporterf	Drug and lipid			[21]

^amethods except for MS,

^bglycopeptide,

^ccytidine 2′monophosphate,

^dthe soluble domain of human growth hormone receptor,

^eneutral nonpolar compounds from the compound collection at Biovitrum AB,

^fATPbinding cassette transporter P-glycoprotein.